Edouard Drouhet, 1919 – 2000

Mucous plug examined by light microscopy with KOH

Mucous plug examined by light microscopy with KOH, showing a network of hyaline branching hyphae typical of Aspergillus, from a patient with ABPA.

Corneal scraping stained with lactophenol cotton blue

Corneal scraping stained with lactophenol cotton blue showing beaded septate hyphae not typical of either Fusarium spp or Aspergillus spp, being more consistent with a dematiceous (ie brown coloured) fungus

Corneal scrape with lactophenol cotton blue

Corneal scrape with lactophenol cotton blue shows separate hyphae with Fusarium spp or Aspergillus spp.

Specimens taken from nails

Skin scraping showing filamentous hyphae with blankophor.

A filamentous fungus in the CSF of a patient with meningitis

A filamentous fungus in the CSF of a patient with meningitis that grew Candida albicans in culture subsequently.

Transmission electron micrograph of a C. neoformans

Transmission electron micrograph of a C. neoformans cell seen in CSF in an AIDS patients with remarkably little capsule present. These cells may be mistaken for lymphocytes.

India ink preparation of CSF

India ink preparation of CSF showing multiple yeasts with large capsules, and narrow buds to smaller daughter cells, typical of C. neoformans

Histological staining

PAS-stain. An example of Aspergillus fumigatus.

Histological staining

PAS stain. An example of Aspergillus fumigatus.
(PAS-stained) in a patient with chronic granulomatous disease showing a 45 degree branching hypha within a giant cell. Rather bulbous hyphal ends are also seem, which is sometimes found inAspergillus spp. infections, histologically. (x800)