Laboratory protocols

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Protocol overviews
Three Decades of Fungal Transformation: Key Concepts and Applications
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation
Cell Component Extraction
Transport Assays in Aspergillus nidulans
Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans
Assaying antifungal levels
Posaconazole Antifungal Drug Level
Simple HPLC Method for the Determination of Caspofungin in Human Plasma
Bioassay for Determining Voriconazole Levels in Blood
Susceptibility testing
Susceptibility Testing Methods of Antifungal Agents
EUCAST: MIC distributions and ECOFFs
Susceptibility testing of yeasts
EUCAST: Susceptibility testing in moulds
EUCAST: QC Tables for antifungal susceptibility testing
Sampling Aspergillus spores
How to validate an automated colony counter
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
PCR
Differentiation of Fungi Using Hybridization Probes on the LightCycler®
Aflatoxins
Measurement of Aflatoxins Using Capillary Electrophoresis
Model systems: in vivo
Animal Models to Evaluate Anti-infective Pharmacodynamics
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
Assaying mycotoxins
Immunochemical Method for Cyclopiazonic Acid
Immunochemical Method for Cyclopiazonic Acid
Determination of optimum growth conditions for gliotoxin production by Aspergillus fumigatus and development of a novel method for gliotoxin detection.
Genetics
Multi-Copy Genetic Screen in Aspergillus nidulans
Preparation of amphotericin paste
Protocol for aseptic preparation of amphotericin B paste for instillation into pulmonary cavity to treat inoperable aspergilloma

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