Laboratory protocols

Displaying 51 - 75 of 281
Fungal Molecular Biology
DNA extraction – miniprep from spores for PCR
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Two new easy to use vectors for transformations (FGSC)
A practical method for the preparation of total DNA from filamentous fungi
Improved Vectors for Selecting Resistance to Hygromycin
Preparation of chromosomal DNA in agarose plugs
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
RNA extraction: miniprep
Lambda IIBargem7B
RNA extraction: large scale prep
Model systems: in vitro
Antifungal Combinations
Model systems: in vivo
Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
Murine Model of Invasive Aspergillosis
Growth and Storage
A list of Aspergillus mutations and how to deal with them
PCR
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus
whole-cell screening
Natural Products and Antifungal Drug Discovery
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Aflatoxins
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus
Genetics
Evaluation of the Disease Liability of CFTR Variants
Enzyme synthesis
Enzymatic Synthesis of α-Butylglucoside in a Biphasic Butanol-Water System Using the α-Transglucosidase from Aspergillus niger
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Species/Strain identification
Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex® xMAP Technology

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