Laboratory protocols

Displaying 76 - 100 of 281
Fungal Molecular Biology
DNA extraction – miniprep from spores for PCR
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Two new easy to use vectors for transformations (FGSC)
A practical method for the preparation of total DNA from filamentous fungi
Improved Vectors for Selecting Resistance to Hygromycin
Preparation of chromosomal DNA in agarose plugs
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
RNA extraction: miniprep
Lambda IIBargem7B
RNA extraction: large scale prep
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
Aflatoxins
A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection
PCR
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus
Growth and Storage
A list of Aspergillus mutations and how to deal with them
Detection in clinical samples
Protocol for homogenisation of CF sputum samples
Susceptibility testing
Screening for Antibacterial, Antifungal, and Anti quorum Sensing Activity
Biotyping
Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Protein methods
Targeted Gene Disruption in Koji Mold Aspergillus oryzae
Post Antifungal Effects (PAFE)
Postantifungal Effect Methods
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Assaying antifungal levels
Posaconazole Antifungal Drug Level
Model systems: in vivo
Murine Model of Invasive Aspergillosis

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