Random subclone generation – Microcentrifuge tube transformation

ID: 67

Group:

General Molecular Biology

Prepared by:

Bruce A. Roe (broe@uoknor.edu)

Detail:

Microcentrifuge transformations are recommended when a single plasmid is being retransformed or for qualitative transformation experiments. Shotgun cloning experiments should be transformed using the large scale transformation, since the objective is to efficiently obtain transformation of hundreds of distinct recombinant plasmids.

Year prepared: 1990

Date uploaded: 2010-02-26 14:32:11

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