Random subclone generation – DNA ligation

ID: 62

Group:

General Molecular Biology

Prepared by:

Bruce A. Roe (broe@uoknor.edu )

Detail:

DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vector in the presence of buffer, rATP, and T4 DNA ligase (10,11).

Year prepared: 2009

Date uploaded: 2010-02-26 14:31:03

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