Random subclone generation – Competent cell preparation

ID: 63

Group:

General Molecular Biology

Prepared by:

Bruce A. Roe (broe@uoknor.edu )

Detail:

For preparation of competent bacterial cells (14), a glycerol cell culture stock of the respective E. coli strain is thawed and added to 50 ml of liquid media. This culture then is preincubated at 37degC for 1 hour, transferred to an incubator-shaker, and is incubated further for 2-3 hours. The cells are pelleted by centrifugation, resuspended in calcium chloride solution, and incubated in an ice-water bath.

Year prepared: 2009

Date uploaded: 2010-02-26 14:31:03

url:

Laboratory Protocols