Protoplast Transformation of Filamentous Fungi

Detail:

Series: Methods in Molecular Biology | Volume: 638 | Pub. Date: Jun-01-2010 | Page Range: 3-19 | DOI: 10.1007/978-1-60761-611-5_1

Year prepared: 2010

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

Laboratory Protocols

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