Year prepared: 2012
With the completion of genomes of various Aspergillus species, large-scale genome-wide expression studies can be carried out. Genomics, however, is more powerful and efficient when combined with genetics. A multi-copy-based gain-of-function screen is a complementary method to loss-of-function genetic screen and can identify novel genes that may not be easily identifiable through loss-of-function-type screens. Particularly, gain-of-function genetic screens would identify novel activators or repressors of fungal development and secondary metabolism. Here, we describe a working protocol for the identification of novel regulators in Aspergillus nidulans.
url: ProtocolLaboratory Protocols
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Title
Type
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Assaying antifungal levels
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Fungal Molecular Biology
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Fungal Molecular Biology
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Detection in clinical samples
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More protocol collections
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Methods for DNA sequencing
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Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
Fungal Molecular Biology