Detail:
Series: Methods in Molecular Biology | Volume: 1147 | Pub. Date: Aug-02-2014 | Page Range: 187-201 | DOI: 10.1007/978-1-4939-0467-9_13
Year prepared: 2014
Fungi are able to grow as a single-species or a more complex biofilm attached to inert surfaces (catheters…) or tissues (lung…). This last form is a microbial niche which must be considered as a major risk factor of developing a human fungal infection. Nowadays, only a few therapeutic agents have been shown to be active against fungal biofilms in vitro and/or in vivo. So there is a real need to find new anti-biofilm molecules. Here we describe in detail some rapid, 96-well microtiter plate-based methods, for the screening of compounds with anti-biofilm activity against Candida spp. yeasts. Two approaches will be considered: prophylactic or curative effects of the tested compounds by producing biofilms on two supports – polystyrene well surfaces and catheter sections.
url: Access via Springer ProtocolsLaboratory Protocols
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Title
Type
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Fungal Molecular Biology
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Methods for DNA sequencing
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Planar liquid chromatography
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Model systems: in vivo
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Protein methods
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Secondary metabolites