In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods

Detail:

Series: Springer Protocols Handbooks | Year: 2014 | Page Range: 275-291 | DOI: 10.1007/978-1-4614-8636-7_17

Year prepared: 2014

Researchers have conducted tests for antimicrobial activity of natural products by several methods, and a huge collection of results has been generated. However, the lack of standardization of basic methods of investigation has led to accumulation of non-useful data. The diversity of protocols has created divergences among specialists, and often different results are obtained with the same plant extract. Although antimicrobial tests for natural products have not been standardized by regulatory agencies, in this chapter, we recommend the use of some technical parameters for susceptibility testing reviewed by the Clinical and Laboratories Standards Institute.

Laboratory Protocols

Select from:Additional methods & referenceAflatoxinsAssaying antifungal levelsAssaying mycotoxinsAztec Labs Diagnostic testing ProtocolsBiochemical CalculatorsBiofilmBiotypingCell Component ExtractionClinical BreakpointsCollection of Clinical MaterialCosmidsDetection in clinical samplesDNA extractionEnzyme synthesisFungal Molecular BiologyGeneral Molecular BiologyGeneticsGrowth and StorageHistologyMethods for DNA sequencingMicroscopyModel systems: in vitroModel systems: in vivoMore protocol collectionsPCR  -RT-qPCRPhagocytosisPlanar liquid chromatographyPost Antifungal Effects (PAFE)Preparation of amphotericin pasteProtein methodsProtocol overviewsQuality control in mycologySampling Aspergillus sporesSecondary metabolitesSpecies/Strain identification  -In situ hydridizationStandard Operating Procedures:National Aspergillosis CentreSusceptibility testingVeterinaryVideo Protocols at the FGSCWhole blood fractionationwhole-cell screening