HPLC assay method for determining voriconazole levels in serum or plasma

Group:

Detection in clinical samples

Prepared by:

Subhosmito Chakraborty, Brian G Keevil

Detail:

Voriconazole is a broad spectrum antifungal agent used in the treatment of invasive fungal infections such as those caused by Aspergillus and Candida, particularly respiratory infections. It is the drug of choice for invasive aspergillosis. Voriconazole has an extensive list of drug interactions and is metabolized at different rates in patients with cytochrome P450 polymorphisms. Metabolism is fast in children but slow in the elderly. Pharmacokinetics is non-linear in adults and adolescents, and linear in children. It has been shown that there is a risk of therapeutic failure in levels lower than 1.3 mg/L and risk of toxicity in levels greater than 5.7 mg/L. There is strong evidence that therapeutic drug monitoring of patients is beneficial. Titration of doses, based on serum or plasma trough levels, has been suggested.
Compared to bioassays, high performance liquid chromatography (HPLC) is a reliable and robust platform. Tandem mass spectrometry, which is considered as the gold standard, is costly, requires specialized training and not widely available in routine Biochemistry laboratories.

Year prepared: 2014

Date uploaded: 2014-07-02

url:

Laboratory Protocols