HPLC assay method for determining Amphotericin B levels in serum and tissue specimens

ID: 16

Group:

Assaying antifungal levels

Prepared by:

Dr Elizabeth Johnson

Detail:

Monitoring of serum concentrations of amphotericin B during treatment is seldom indicated. The optimum serum concentrations of the drug for particular fungal infections have not been determined. Although amphotericin B is nephrotoxic toxicity is determined by monitoring kidney function and higher blood levels do not lead to greater renal impairment.

Year prepared: 2000

Date uploaded: 2010-02-26 14:39:24

url:

Laboratory Protocols

Showing 10 posts of 364 posts found.

Title

Type

Superoxide assay (Phagocytes)

Phagocytosis
Purification of neutrophils

Whole blood fractionation
EUCAST Technical Note on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia-forming moulds

Susceptibility testing
Microsatellite typing for Aspergillus fumigatus

Species/Strain identification
SOP’s for the Preparation of Aspergillus test strains for inhalation pulmonary aspergillosis studies including : Processing tissue samples for PCR , Galactomannan; DNA extraction for quantitative real-time PCR , and of tissue fungal burden

Model systems: in vivo
Colony identification key: A key for the identification of species via colony morphology on standard media.

Species/Strain identification
Eosinophilic fungal rhinosinusitis – Clinical material collection and culture technique

Collection of Clinical Material
Preparation of Solid Tissue for Aspergillus Galactomannan

Detection in clinical samples
A rapid method for generating gene deletions in Aspergillus fumigatus

General Molecular Biology
Detection of medically important fungi using PCR

Detection in clinical samples

Laboratory Protocols

Title

Type