Detail:
Series: Methods in Molecular Biology | Volume: 1508 | Pub. Date: Oct-08-2016 | Page Range: 267-279 | DOI: 10.1007/978-1-4939-6515-1_15
Year prepared: 2016
PCR-based detection of fungal pathogens offers a sensitive and specific tool for the diagnosis of invasive fungal infections. A large variety of different clinical specimen types can be used as original material. However, certain precautions, in addition to the published MIQE guidelines, need to be taken to prevent contaminations from airborne fungal spores and PCR reagents. In addition, the European Aspergillus PCR Initiative (EAPCRI) recently defined standards for Aspergillus PCR, following these recommendations leads to superior sensitivity. The combination of fungal PCR with the galactomannan ELISA assay increases the sensitivity for the detection of Aspergillus DNA from blood, compared to a single assay only. (Methods in Molecular Biology)
url: Access via Springer ProtocolsLaboratory Protocols
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Title
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Assaying antifungal levels
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Fungal Molecular Biology
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Fungal Molecular Biology
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Detection in clinical samples
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Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
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