Determination of Plasmid Copy Number in Yeast

Detail:

Series: Methods in Molecular Biology | Volume: 53 | Pub. Date: Feb-19-1996 | Page Range: 193-203 | DOI: 10.1385/0-89603-319-8:193

Year prepared: 1996

Autonomously replicative plasmids are widely employed as vectors for the expression of cloned genes in yeast (1). A number of different kinds of such plasmid vectors exist, which fall into three classes: ARS (autonomously replicating sequence) based plasmids, 2-µm-based plasmids, and ARS plasmids containing a centromere.

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