Culture-Based Techniques

Detail:

Series: Methods in Molecular Biology | Volume: 1508 | Pub. Date: Oct-08-2016 | Page Range: 195-207 | DOI: 10.1007/978-1-4939-6515-1_10

Year prepared: 2016

The detection of fungal elements and their characterization in patient specimens provides fundamental information. Culture-based methods, though often slow, may yield the specific etiological agent, and may allow susceptibility testing to be performed. Proper collection and transportation of the specimen is essential. Particularly, sterile materials are important for diagnosis of invasive fungal infections.
Therefore, culture and direct microscopy should be performed on all suitable clinical specimens when fungal disease is suspected. Numerous different media for culturing and identifying fungi are available, and those important for diagnosing mycoses as well as the most important staining methods for direct microscopy are described.

Laboratory Protocols

Select from:Additional methods & referenceAflatoxinsAssaying antifungal levelsAssaying mycotoxinsAztec Labs Diagnostic testing ProtocolsBiochemical CalculatorsBiofilmBiotypingCell Component ExtractionClinical BreakpointsCollection of Clinical MaterialCosmidsDetection in clinical samplesDNA extractionEnzyme synthesisFungal Molecular BiologyGeneral Molecular BiologyGeneticsGrowth and StorageHistologyMethods for DNA sequencingMicroscopyModel systems: in vitroModel systems: in vivoMore protocol collectionsPCR  -RT-qPCRPhagocytosisPlanar liquid chromatographyPost Antifungal Effects (PAFE)Preparation of amphotericin pasteProtein methodsProtocol overviewsQuality control in mycologySampling Aspergillus sporesSecondary metabolitesSpecies/Strain identification  -In situ hydridizationStandard Operating Procedures:National Aspergillosis CentreSusceptibility testingVeterinaryVideo Protocols at the FGSCWhole blood fractionationwhole-cell screening