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Creating a Gene Replacement Construct (Cramer Lab)

ID: 156

Group:

Fungal Molecular Biology

Prepared by:

Robert Cramer Jnr.

Detail:

Adapted for use in A. fumigatus

Year prepared: NULL

Date uploaded: 2010-09-23 16:25:18

File upload: https://www.aspergillus.org.uk/wp-content/uploads/2015/08/Cramer_KO_constructs.pdf

Laboratory Protocols

Showing 10 posts of 364 posts found.

Title

Type

Voriconazole II Assay

Assaying antifungal levels
Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of 5 azole antifungals and 1 active metabolite

Assaying mycotoxins
Northern blot

Fungal Molecular Biology
SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption

Fungal Molecular Biology
ELISA for Aspergillus fumigatus in dust (CDC method)

Detection in clinical samples
AspGD

More protocol collections
Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue

Histology
A Multi-Step Chromatographic Strategy to Purify Three Fungal Endo-β-Glucanases

Protein methods
Library Preparation and Data Analysis Packages for Rapid Genome Sequencing

Methods for DNA sequencing
Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi

Fungal Molecular Biology

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