Concentration of DNA by ethanol precipitation

ID: 48

Group:

General Molecular Biology

Prepared by:

Paul Bowyer

Detail:

Typically, 2.5 – 3 volumes of an ethanol/acetate solution is added to the DNA sample in a microcentrifuge tube, which is placed in an ice-water bath for at least 10 minutes. Frequently, this precipitation is performed by incubation at -20C overnight (1). To recover the precipitated DNA, the tube is centrifuged, the supernatant discarded, and the DNA pellet is rinsed with a more dilute ethanol solution. After a second centrifugation, the supernatant again is discarded, and the DNA pellet is dried in a Speedy-Vac.

Year prepared: 2009

Date uploaded: 2010-02-26 14:11:47

url:

Laboratory Protocols