AspGD

http://www.aspergillusgenome.org/Methods.shtml

– Osmani AH, et al. (2006) Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique. Nat Protoc 1(5):2517-26
– Szewczyk E, et al. (2006) Fusion PCR and gene targeting in Aspergillus nidulans. Nat Protoc1(6):3111-20
– Todd RB, et al. (2007) Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction. Nat Protoc 2(4):811-21
– Todd RB, et al. (2007) Genetic manipulation of Aspergillus nidulans: heterokaryons and diploids for dominance, complementation and haploidization analyses. Nat Protoc 2(4):822-30
– Liu HL, et al. (2010) Single step affinity purification for fungal proteomics. Eukaryot Cell. 2010 Apr 2. [Epub ahead of print]

Laboratory Protocols

Select from:Additional methods & referenceAflatoxinsAssaying antifungal levelsAssaying mycotoxinsAztec Labs Diagnostic testing ProtocolsBiochemical CalculatorsBiofilmBiotypingCell Component ExtractionClinical BreakpointsCollection of Clinical MaterialCosmidsDetection in clinical samplesDNA extractionEnzyme synthesisFungal Molecular BiologyGeneral Molecular BiologyGeneticsGrowth and StorageHistologyMethods for DNA sequencingMicroscopyModel systems: in vitroModel systems: in vivoMore protocol collectionsPCR  -RT-qPCRPhagocytosisPlanar liquid chromatographyPost Antifungal Effects (PAFE)Preparation of amphotericin pasteProtein methodsProtocol overviewsQuality control in mycologySampling Aspergillus sporesSecondary metabolitesSpecies/Strain identification  -In situ hydridizationStandard Operating Procedures:National Aspergillosis CentreSusceptibility testingVeterinaryVideo Protocols at the FGSCWhole blood fractionationwhole-cell screening