TYPING OF ASPERGILLUS FUMIGATUS BY RAPD
The European Research Group on Biotype and Genotype of Aspergillus 67 strains from 8 cases of patients with aspergillosis and from their environment in 3 different European regions were collected by members of the network. A complete medical file was available for each patient. Strains were typed by RAPD (Random Amplified Polymorphic DNA) using the arbitrary single primer NS3 from the fungal ribosomial gene 18S subunit under low stringency conditions. Results showed: 1) 4 cases with several isolates from the same clinical sample : all the isolates from 3 patients (5 isolates per patient) revealed identical RAPD patterns, while for the fourth case 2 types (on 9 isolates) were found 2) 2 cases with everal isolates from different sites collected at different times intervals: in the first case 9 isolates from the patient belonged to 3 RAPD types, and in the second case all the 13 isolates remained identical until autopsy. 3) Relation with environmental isolates : in one case (9 isolates) a neurosurgical patient undoubtfully became contaminated by an environmental source, while in the second case (13 isolates) 4 isolates from the patient were identical and showed RAPD type different from those occuring in hospital environment and dwelling.
Full conference title:
The 2nd Meeting of the European Confederation of Medical Mycology