DETECTION OF ANTI-ASPERGILLUS FUMIGATUS ANTIBODIES BY IMMUNOBLOT AND ELISA ASSAY IN PATIENT WITH ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS
Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung; disease occuring as a complication of asthma in patients colonized with Aspergillus fumigatus (Af). The diagnosis of ABPA is based on clinical criteria of wheezing and pulmonary infiltrates associated with the laborat -ory criteria of elevated serum IgE, eosinophilis, and increased IgE and IgG anti-Af antibodies. In this study ELISA and immunoblot assay were used to detect IgE and IgG anti-Af in patients with ABPA and control group, including allergic patients with asthma without allergy to A. fumigatus. Patients with ABPA (n=12) showed significant elevated level: of IgE and IgG anti-Af by both techniques. However, allergic patients, with asthma without A.fumigatus allergy (n=19) did not have detectable amounts of Af-antibodies. The range of molecular weight of Af-proteins, which responsded to Af-IgG and IgE antibodies, were from 15 to 128 kDa.
Full conference title:
The 2nd Meeting of the European Confederation of Medical Mycology