The Cross-Pathway Control of the Opportunistic Pathogen Aspergillus fumigatus Cloning and Characterisation of its Transcriptional Activator CPCA.
The filamentous saprophyte Aspergillus fumigatus accounts for the majority of aspergillosis incidents, which represent a severe threat mainly to immunocompromised individuals. Detailed knowledge on factors contributing to pathogenicity of this opportunistic pathogen is scarce with only few determinants identified. Nutritional requirements and metabolic features rendering the fungus infective have not been investigated in great detail. We have focused on the Cross-Pathway Control (cpc) system of A. fumigatus, a global regulatory network acting on amino acid biosynthesis to counteract conditions of starvation or imbalance. The cpcA locus encoding the transcriptional activator protein of the system was identified and characterised. Its gene product represents a highly conserved protein that is the functional orthologue of Gcn4p, the yeast master regulator of gene expression upon starvtion and stress. Generation of cpcA deletion mutants revealed its central role for the cpc response of this fungus upon amino acid starvation. Next steps aim at the impact of the cpc system on pathogenicity of A. fumigatus, the current status of the project will be presented.
Fungal Genet. Newsl. 50 (Supl):abstract
Full conference title:
22nd Fungal Genetics Conference