Antimicrobial peptides with antifungal activity

Serge Ruden2, Ralf Mikut2, Norio Takeshita2, Daniel Mania2, Reinhardt Fischer2, KAI HILPERT1 ,2

Abstract: 

As recently discussed in Nature Reviews Microbiology 11, 146 (March 2013), “there are few public health issues of potentially greater importance for society than antibiotic resistance”. Novel antibiotics are urgently needed, but the drug development pipelines are “dry”. Antimicrobial peptides (AMPs), are substances that can effectively kill a broad range of life threatening multidrug-resistant bacteria and fungi. However, despite great hopes for novel AMP drugs, such drugs are still rare. To accelerate drug development we studied the connection of antibacterial activity and cytotoxicity. To accomplish this, we determined for 3,000 different 9meric peptides the concentration that killed bacteria, Rel(AB)IC75, and compared it to the concentration that lead to 75% hemolysis of human red blood cells Rel(H)IC75. Hemolytic activity was cross-validated in vitro and in vivo using cell culture and zebrafish larvae. In this process we discovered peptides that were active against Candida albicans and Aspergillus nidulans. Fluorescent labelled peptides showed that within 4 hours the peptides accumulated within the cytosol of Aspergillus nidulans, indicating an internal target

2013

abstract No: 

SU02Mo1000

Full conference title: 

Microbiology Society Annual Conference