Fungal biofilm infections have become increasingly recognised as a significant clinical problem. One of the major reasons behind this is the impact that these have upon treatment, as antifungal therapy often fails and surgical intervention is required. This places a large financial burden on health care providers. Pathogenic fungi, such as Candida albicans and Aspergillus fumigatus, are armed with an arsenal of adaptive mechanisms that enable them to survive and proliferate within the host in the presence of antifungal agents. The aim of this presentation is to discuss the variety of adaptive resistance mechanisms used by these organisms, including extracellular matrix (ECM), efflux pump activity, extracellular DNA, stress responses and persister cells. Our recent insights into the processes and mechanisms involved in biofilm antifungal resistance will be presented.