Ref ID: 18291
Author:
. Gsaller
, M. Eisendle
, B. Lechner
, M. Schrettl
,D. Mueller
, H. Lindner
, B. Sarg
, S. Geley
, H. Haas
Author address:
Division of Molecular
Biology, Biocenter, Medical University of Innsbruck, Austria;
Division of Clinical Biochemistry, Biocenter, Medical University of
Innsbruck, Austria;
Division of Molecular Pathophysiology, Biocenter, Medical University o
Full conference title:
Asperfest 9
Abstract:
Iron is an essential element for all eukaryotes but its excess is deleterious. Iron homeostasis results from tight regulation of iron
acquisition and iron storage. A. fumigatus produces the extracellular siderophores triacetylfusarinine C (TAFC) and fusarinine C
(FSC) for iron uptake and the intracellular siderophores ferricrocin (FC) and hydroxyferricrocin for iron distribution and storage.
Siderophore biosynthesis is important for the adaptation to iron starvation and therefore crucial for virulence. Intracellular iron excess
has been shown to increase the content of FC-chelated iron and the expression of AFUA_4g12530, termed CccA, which shows
similarity to the vacuolar iron importer Ccc1 of S. cerevisiae. These data indicate a role of both the vacuole and FC in iron
detoxification. Green fluorescence protein-tagging confirmed localization of CccA in the vacuolar membrane. During high iron
conditions genetic inactivation of CccA impaired growth, in particular in combination with derepressed iron uptake due to deficiency
in the iron regulator SreA. In contrast, overproduction of CccA increased iron resistance. Inactivation of FC biosynthesis did not affect
iron resistance. Lack of FC, CccA and in particular both, increased the cellular content of iron chelated by FSC/TAFC breakdown
products. A delayed release of iron from FSC/TAFC degradation products might represent another iron detoxifying mechanism. Our
data indicate that vacuolar rather than FC-mediated iron storage is the major iron detoxifying mechanism of A. fumigatus.
Abstract Number: 41)
Conference Year: 2012
Link to conference website: NULL
New link: NULL
Conference abstracts, posters & presentations
-
Title
Author
Year
Number
Poster
-
v
T. Peláez*, B. Gama, L. Alcalá, E. Bunsow, M. Valerio, A. Fernández-Cruz, J. Guinea, E. Bouza.
2012
P824
n/a
-
v
Zaragoza R1, Pemán J2, Cantón E2, Camarena J1, Ayats J3, Torroba L4, Navarro D5, Royo G6, Echevarria J7,
Martinez J8, Guna R9, Ramirez I10, and the Spanish FUNGEMYCA group.2012
P823
n/a
-
v
P. Muñoz*, I. Cerón, M. Valerio, J. Palomo, A. Villa, A. Eworo,
J. Guinea, I.A. González, E. Bouza2012
P804
n/a
-
v
Pauline Krijgsheld,
A.F. Maarten Altelaar,
Harm Post,
Jeffrey H. Ringrose,
Wally H. Müller,
Albert J.R. Heck,
Han. A.B. Wösten2012
PR8.11
n/a
-
v
M. Nagao*, T. Saito, G. Hotta, S. Doi, M. Yamamoto, Y. Matsumura,
A. Matsushima, Y. Ito, S. Takakura, S. Ichiyama2012
NULL
n/a
-
v
R. Patel*, B. Saberwal, S. Quan, A. Gonzalez-Ruiz
2012
NULL
n/a
-
v
M.L. Moretti, P. Trabasso, L. Lyra, R. Fagnani, M.R. Resende,
L.F. Bachur, L.G.O. Cardoso*, A.Z. Schreiber2012
P783
n/a
-
v
C. Bulut*, Y. Kurtoglu Gul, C. Ataman Hatipoglu, S. Yagci,
A.P. Demiro¨2012
NULL
n/a
-
v
Sehnaz ALP, Sevtap ARIKAN-AKDAGLI, Dolunay GULMEZ, Sibel ASCIOGLU, Omrum UZUN, Murat AKOVA
2012
P777
n/a
-
v
S. Geith (1), F. Saghrouni (2), J. Ben Abdeljelil (2), I. khammari (2), W. Bannour (1), Y. Ben Youssef (3), S. Ben Salma (1), A. Khelif (3), M. Ben Said M (2), M. Njah (1), A. Fathallah (2).
2012
P775
n/a