New promoters to improve heterologous protein production by Aspergillus vadensis

Ref ID: 18312

Author:

Ourdia BOUZID(1,2), Chema Messlaki(1,2), Andreas Spelberg(1,2), Helena M Culleton(2,3), Vincent A McKie(3), Barry V
McCleary(3), Ronald P de Vries(1,2)

Author address:

(1) Microbiology & Kluyver Centre for Genomics of Industrial Fermentation, Utrecht
University, Utrecht, The Netherlands (2) CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands (3) Megazyme
International Ireland Ltd, Wicklow, Ireland

Full conference title:

Asperfest 9

Abstract:

Aspergillus species have always been used as producers of homologous and heterologous proteins. Although Aspergillus niger is most
commonly used, a close relative of this species, Aspergillus vadensis, has been suggested as a better candidate As this species
produces very low levels of extracellular proteases and does not acidify the medium [1]. It has also been shown that this species can
produce higher levels of A. niger FaeB than A. niger itself [2] and can produce a basidiomycete esterase that could not be produced by
A. niger [3]. To further improve protein production in A.vadensis, six new constitutive promoters have been selected from A. niger and
tested in A.vadensis. in comparison to the gpdA promoter [4]. selected gene encoding a secreted arabinofuranosidase was used as a
reporter for protein production. Based on activity all seven promoters activate the expression of the abf, some at higher levels than
gpdA. [1] de Vries et al. Appl Environ Microbiol 2004, 70: 3954-3959 [2] Alberto et al. Lett Appl Microbiol 2009, 49: 278282 [3]
uranov et al. Arch Microbiol 2009, 191: 133-140 [4] Punt et al. GENE. 1990, 93 :101-109

Abstract Number: 63)

Conference Year: 2012

Link to conference website: NULL

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