HLA genotyping in ABPA and non-ABPA greek cf patients

Author:

Maria Noni (GR)

Abstract:

Background: Allergic bronchopulmonary aspergillosis (ABPA) is a complex pulmonary disorder characterized by a hypersensitivity reaction to Aspergillus fumigatus, and almost always seen in patients with cystic fibrosis (CF) and asthma. Few studies investigated the implication of HLA class II antigens in the genetic background of ABPA. Our aim was to investigate HLA class II alleles in Greek CF patients with ABPA.
Methods: HLA genotyping for DRB1 and DQB1 was performed in 43 CF patients (32 CF patients with ABPA and 11 CF patients without ABPA) aged 2 to 19 years old. Statistical analysis included chi-square test and/or Fischer’s exact test.
Results: Our study population included 24 male and 19 female CF patients. Thirty-four different DRB1 genotypes were detected. Genotypes DRB1*1101+DRB1*1104 and DRB1*1104+DRB1*1104 were the most prevalent (14%). Twenty different DQB1 genotypes were detected and DQB1*0301+DQB1*0301 was the most common one (20.9%). DRB1*11 and DRB1*16 alleles were the most frequently detected (39.5% and 12.8%, respectively). Regarding DQB1 alleles, DQB1*03 and DQB1*05 were the most frequently detected (45.3% and 27.9%, respectively).
Among ABPA patients, 26 different DRB1 genotypes were detected and DRB1*1101+DRB1*1104 and DRB1*1104+DRB1*1104 were the most frequent (18.8%). Fourteen different DQB1 genotypes were detected and DQB1*0301+DQB1*0301 was the most common (21.9%). DRB1*11 and DQB1*03 alleles were the most frequently detected (39.1% and 46.9%, respectively) but were not found significantly different between ABPA and control CF patients (p=0.88 and p=0.63, respectively). DRB1*1501 was detected only in two patients with ABPA, while DQB1*0201 was detected only in three control CF patients.
Thirty-four different DRB1-DQB1 combinations were detected. The most common one was DRB1*11-DQB1*03 (29.1%%), followed by DRB1*16-DQB1*05 (8.7%). Both genotypes were not found statistically significant between ABPA and control CF patients (p=0.93 and p=0.47, respectively).
Conclusions: According to the literature, HLA-DRB1*1501 and HLA-DRB1*1503 confer the highest risk of developing ABPA, whereas HLA-DQB1*0201 provides relative protection against the development of ABPA. The frequency of HLA alleles differs in our study group. This difference could be attributed to the small sample size. Further studies are necessary to clarify the implication of HLA class II antigens in the genetic background of ABPA.

Abstract Number: 80

Conference Year: 2024

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