Ref ID: 17749
Author:
F. Haghighi*, S. Roudbar Mohammadi, E. Farahbakhsh,
M. Eskandari
Author address:
(Tehran, IR)
Full conference title:
22nd European Congress of Clinical Microbiology and Infectious Diseases
Abstract:
Objectives: Biofilms represent the most common type of microbial
growth in nature and are critical to development of many infections in
human. Recently it has been reported that 65% of all human infections
involve biofilms. Nowadays Candida albicans has more effective role
among nosocomial pathogens due to proper potential for biofilm
formation. Using a novel method in field of inhibitory formation of C.
albicans biofilm and elimination of fungal mass over surfaces is
valuable for controlling infections. In this study antifungal effect of
ZnO/Ag nanoparticles were evaluated on standard strain of C.
albicans.
Methods: ZnO nanoparticles and Ag were synthesized through the solgel
method by Zinc Acetate Dehydrate [Zn(CH3COOH)2.2H2O] and
AgNo3 as precursor respectively. The morphology properties of the
products were analyzed by using Scanning Electron Microscopy
(SEM). Biofilm of standard strain of C. albicans (ATCC 10231) was
developed on flat-bottomed 96-well microtiter plates and antifungal
effects of ZnO/Ag nanoparticles were evaluated by using standard
accurate method, CCK8 (Cell Counting Kit 8) as colorimetric assay for
measuring metabolic activity of sessile cells within the biofilm as it
relates to cellular viability. Data were analyzed using t-test statistical
method and SPSS software.
Abstract Number: P815
Conference Poster: y
Conference Year: 2012
Link to conference website: NULL
New link: NULL
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