Ref ID: 18295
Author:
J.M. van Munster
, B.M. Nitsche
, A.F.J. Ram
, R.M. van der Kaaij
, L. Dijkhuizen
, M.J.E.C. van der Maarel
Author address:
Microbial
Physiology , University of Groningen, The Netherlands.
Molecular Microbiology, Leiden University, The Netherlands
J.M.van.Munster@rug.nl
Full conference title:
Asperfest 9
Abstract:
Aging and stress conditions during industrial fermentations of Aspergillus niger may lead to the initiation of
sporulation and autolysis, during which glycoside hydrolases (GH) can modify the cell wall structure. A full understanding of
processes taking place during aging and nutrient starvation contributes to the identification of strategies to increase fermentation
efficiency. Using transcriptome analysis, we identified glycoside hydrolases of A. nigerproduced by aging and nutrient starved
mycelium. Two enzymes, putative chitinases, were heterologously expressed, purified and characterised to gain more insight in their
physiological and metabolic function. CfcA releases mainly chitobiose from the non-reducing end of chitin oligosaccharides and from
chitin present in the fungal cell wall. CfcI is capable of hydrolysing chitotriose and longer chitin oligosaccharides. CfcI functions by
cleaving off monomers, possibly in a processive mode, acting on the reducing end of the oligosaccharide substrates. To the best of our
knowledge, this activity has not been reported before for fungal chitinases of glycoside hydrolase family 18. We acknowledge
AgentschapNL for funding IOP Genomics project IGE07008
Abstract Number: 46)
Conference Year: 2012
Link to conference website: NULL
New link: NULL
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