Author:
Guan Pang1 Jian Zhang1 Feng Cai1 Hong Zhu1 Irina S. Druzhinina2 Qirong Shen1
Author address:
1 Jiangsu Key Lab for Organic Waste Utilization and National Engineering Research Center for Organic-Based Fertilizers, Nanjing Agricultural University, Nanjing, China
2 Microbiology and Applied Genomics Group, Research Area Biochemical Technology, Institute of Chemical, Environmental and Biological Engineering, TU Wien, Vienna, Austria
Full conference title:
The 14th European Conference on Fungal Genetics, Haifa, Israel
Date: 13 March 2018
Abstract:
Harzianolide, a secondary metabolite from a group of butenolides, is secreted by Trichoderma species (Hypocreales, Ascomycota) from the Harzianum Clade. Previous studies suggest that this compound is involved in plant growth promotion and it also triggers plant defense responses. In this research, we have used HPLC/MSMS and NMR to isolate and identify harzianolide from the cultivation broth of Trichoderma guizhouense NJAU 4742 (Harzianum Clade) grown in potato dextrose broth. We also used the RNA deep sequencing and gene deletion experiment to predict and verify the cluster of secondary metabolite producing genes that are involved in harzianolide synthesis. Interestingly, the ∆pks7 mutant of T. guizhouense NJAU 4742 lacking the gene encoding polyketide synthase orthologous to the reducing PKS7 (fumonisins) sensu Baker et al. (2012), resuscitates the biosynthesis of the two isomers of harzianic acid, another plant growth promoting metabolite of Trichoderma that also has its antifungal activity. Consequently, the mutant is able to suppress the growth of several other fungi including pathogens of plants. To investigate the synthetic pathway for harzianolide, we used Saccharomyces cerevisiae BJ5464-npgA as a host for the heterologous expression of this PKS7. Thus, the combination of metabolomics and transcriptomic data points to the possible involvement of harzianolide in the regulation of secondary metabolite production in T. guizhouense.
Conference Year: 2018
Link to conference website: IDWeek 2016
Link Conference abstract:
Conference abstracts, posters & presentations
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Title
Author
Year
Number
Poster
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v
Felix Bongomin (UG)
2024
41
n/a
-
v
Khostelidi S1,2, Borzova Y2., Shadrivova O1, Desyatik E2., Karpishchenko S3 ., Charushin A.O4., Avdeenko Y2., Bogomolova T2., Ignatieva S2., Taraskina A.2, Burova S.A5., Vasylieva N.V.2
2024
40
n/a
-
v
Balogun O.J, Ajibola O.O.E., Oladele R.O., Fashanu T.A, Gangneux J.P., Denning D.W
2024
39
n/a
-
v
Su Chen, Ruoyu Li, Wei Chen, Zhe Wan, Wei Liu
2024
38
n/a
-
v
K.A. Marr,1,2 N. Permpalung,1 S. Rajack,1 K. Datta,1 D. Ostrander,1 J.F. Staab,1 K. Witwer,1 I. Baburina,2 D. Neitzey,2 P. X. Marques,2 R. Aerts,3 J. Maertens,3 D. Sheppard,4 J.R. Wingard,5 and S. X. Zhang,1
2024
37
n/a
-
v
Walshe. K, Dwyer. M and Doyle.S (IE)
2024
36
n/a
-
v
Jiří Houšť1, Radim Dobiáš2, Dominika Luptáková1, Andrea Palyzová1, Tomáš Pluháček1, Milan Navrátil3, David A. Stevens4, Rutuja H. Patil1, and Vladimír Havlíček1
2024
35
n/a
-
v
Mragnayani Pandey (IN), Immaculata Xess, Janya Sachdev, Neha Sharad, Sonakshi Gupta, Gagandeep Singh, Renu Kumari Yadav, Bhaskar Rana, Stephen Raj, M Nizam Ahmad, Neha Nityadarshini, Upendra Baitha, Manish Soneja, Shalimar, Bindu Prakash, Kapil Sikka, Purva Mathur, Viveka P Jyotsna, Rakesh Kumar, Naveet Wig, Ashutosh Biswas, Alok Thakar,
2024
34
n/a
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v
Vladimir Havliecek1,2, R Dobias3,4, D Luptakova1, R Patil1,2, J Houst1,2, D Stevens5,6, M Petrik7, A Palyzova1, T Pluhacek1,2
2024
33
n/a
-
v
Sebastien Imbert (FR)
2024
32
n/a