Laboratory protocols

Displaying 26 - 50 of 281
Fungal Molecular Biology
Preparation of chromosomal DNA in agarose plugs
Improved Vectors for Selecting Resistance to Hygromycin
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
RNA extraction: miniprep
Lambda IIBargem7B
RNA extraction: large scale prep
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
RNA extraction: Isolation of mRNA
PCR from spores
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Isolation of RNA from yeast
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Multi-Copy Genetic Screen in Aspergillus nidulans
Real-Time Quantitative PCR, Pathogen Detection and MIQE
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Model systems: in vitro
Antifungal Combinations
Model systems: in vivo
Murine Model of Invasive Aspergillosis
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
Methods for DNA sequencing
Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
whole-cell screening
Natural Products and Antifungal Drug Discovery
Protocol overviews
Fungal Diagnostics: Review of Commercially Available Methods
Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus