Vogel's Minimal Medium

Vogel's 50X salts as given in the Microbial Genetics Bulletin 13:42-43, 1956. In 750 ml. distilled water, dissolve successively with stirring at room temperature:

                              
Na3 citrate, 5 1/2 H20                 150 grams(NOTE, you may substitute 125 g Na3 Citrate 2 H2O)
KH2PO4, anhydrous                      250 grams
NH4NO3, anhydrous                      100 grams
MgSO4, 7 H20                            10 grams
CaCl2, 2 H20                             5 grams
Trace Element Solution (see below)       5 ml.
Biotin Solution (see below)              2.5 ml.

The resulting total volume is about 1.00 liter. Chloroform (2 ml. ) is added as a preservative. 
Store at room temperature.

Vogels Medium N (Minimal) is

20 mL Vogel's Salts
15 grams Sucrose
1 liter dH2O

add 15 grams agar for solid medium

The trace element solution (containing citric acid as a solubilizing agent) is made up as follows: In 95 ml. distilled water, dissolve successively with stirring at room temperature:

                  Citric acid, 1 H20         5. 00 grams
                  ZnSO4, 7 H20               5. 00 grams
                  Fe(NH4)2(SO4)2, 6 H20      1. 00 gram
                  CuSO4, 5 H20               0. 25 gram
                  MnSO4, 1 H20               0. 05 gram
                  H3BO3, anhydrous           0. 05 gram
                  Na2MoO4, 2 H20             0. 05 gram

The resulting total volume is about 100 ml. Chloroform (1 ml. ) is added as a preservative, and the trace element solution is stored at room temperature.

The biotin solution is prepared by dissolving 5.0 mg. biotin in 50 ml. distilled water. The solution obtained is dispensed in test tubes and stored in the frozen state.

Year prepared: 

2000