Purification and characterisation of novel recombinant β-glucosidases from Aspergillus

RICHARD AUTA, Paul Hooley, Iza Radecka


An initial bioinformatics analysis of the genome databases for β–glucosidases for Aspergillus has collated the variation in this enzyme class using keyword searches and Blast. Selection of novel candidates for direct gene synthesis for cloning and expression is based on size (short genes), pI (ExPASy – Tools) and novelty. Several Aspergillus strains have been screened using a rapid plate assay based on Congo Red. Selection of candidate strain was based on temperature profile, pH range and carbon source degradation. Potential bacterial donors of cellulose degrading enzymes have also been explored for their expression in fungal hosts. Pichia pastoris systems will be used for enhanced expression of Aspergillus proteins employing fusion PCR of the target gene with an inducible promoter. At the end of this work we hope to generate new β-glucosidases, cloned and analyzed for industrial use to produce biofuel (renewable energies)


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Microbiology Society Annual Conference