Background: Aspergillosis is known to be an airborne infection and the nosocomial infections are associated with constructions and increased dust loads in hospital indoors. Our main object was to find the environmental sources of Aspergillus species causing hospital acquired infections.
Material/methods: The clinical and environmental samplings were performed during 18 months from spring 2010 to summer 2011 in a large educational hospital. A morphological diagnosis was used including media culture, for the first identification of all isolated Aspergillus species. For the RAPD assay, extraction of DNA was performed using manual phenol-chloroform method followed by PCRwith six random primers. The results of RAPD were compared between the clinical Aspergillus isolates and hospital indoor isolates.
Results: Use of RAPD method resulted various differential patterns so that some Aspergillus isolatesfrom the clinical and hospital indoor including the pairs 32 and 45 among 51 Aspergillus pairs, werecompletely matched. Some other Aspergillus pairs: 16, 31 and 237 were not matched. The Aspergillus isolate of pair 32 was obtained from both bronco alveolar lavage and air conditioner as a same RAPDtype of A. niger. Also, the same RAPD type of A. flavuswas isolated from sinus discharge as well as the walls surface.
Conclusions: The hospital sources for the Aspergillus clinical isolates included air conditioners and rooms walls and RAPD-PCR analysis can play a trivial role in finding the hospital sources of Aspergillus clinical isolates.