Media for culture of Aspergillus nidulans are described in a variety of sources.
One authoratitive source is :Barratt et al., 1965 Genetics 52:233-246
Examples from this work commonly used at the FGSC and are described below.
per liter NaNO3 6.0 gm KC1 0.52 gm MgSO4. 7H20 0.52 gm KH2PO4 1.52 gm Adjust pH to ca 6.5 (usually requires 1 ml of 1 N NaOH) (Note: If large quantities of minimal are to be used a 2OX stock solution of the above salts can be prepared.) glucose (dextrose) 10.0 gms 2 ml of Hutner's trace elements For agar add 15.0 gms Difco Agar
Hutner's trace elements
H20 100 ml ZnSO4-7H20 2.2 gm H3BO3 1.1 gm MnCl2-4H20 0.5 gm FeSO4-7H20 0.5 gm CoCl2-6H20 0.16 gm CuS04-5H20 0.16 gm (NH4)M07024-4H20 0.11 gm EDTA* 5.0 gm
To prepare trace elements: Heat to boiling, cool to 60 C, add KOH adjusting pH to 6.5-6.8. Sol'n goes to deep purple after standing for several days. If in titrating pH, pH exceeds 7.0 discard and start over.
*ethylene diamine tetra acetic acid (Disodium salt is easiest)
A Complete medium glucose 2.5% yeast extract 0.5% agar 1.5% Another Complete medium Difco malt extract 2% Bacto-peptone 1% glucose 2% agar 2%
A third alternative is to use Neurospora Complete medium.
Finally, to convert minimal to complete add the following per liter of minimal.
Difco Bacto peptone 4.0 gm Difco Bacto yeast extract 2.0 gm. N-Z-case (Borden, enzyme hydrolyzed casein)2.0 gm vitamin solution* 2.0 ml hydrolyzed nucleic acid 6.0 ml
*vitamin stock solution
per 100 ml H20 Biotin 0.01 gm pyridoxinHCl 0.01 gm thiamineHCL 0.01 gm riboflavin 0.01 gm paba 0.01 gm nicotinic acid (or niacin) 0.01 gm
Store in dark; sterilize by autoclave or better yet by adding a few drops of chloroform.
ribo, ad, meth and phen may require appropriate supplementation)
Cultures are incubated at 37'C.
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