A list of Aspergillus mutations and how to deal with them

Aspergillus nidulans mutants

This list of A. nidulans mutant alleles was compiled by Craig Wilson. It is maintained for archival purposes.

Here is an incomplete compilation of A. nidulans mutants listing names, affected enzymes (or gene functions), linkage assignments, properties of the mutants, and names of corresponding loci in N. crassa. The loci included represent mutants in the FGSC collection.

 

Locus Designation : AcrA 
Linkage group : IIL 
Cultural requirement (property of mutant): Resistant to acriflavine 

 

Locus Designation : aaX 
Linkage group : VIR 
Enzyme (or gene function) : allantoicase 
Cultural requirement (property of mutant): cannot use allantoic acid as nitrogen source 
Corresponding mutation in Neurospora: alc-1 

 

Locus Designation : abaA 
Linkage group : VIIIR 
Cultural requirement (property of mutant): grows on minimal, is aconidial 

 

Locus Designation : acrB 
Linkage group : IIR 
Cultural requirement (property of mutant): resistant to acriflavine 

 

Locus Designation : actA 
Linkage group : IIIL 
Cultural requirement (property of mutant): resistant to cycloheximide (actidione) 

 

Locus Designation : actB 
Linkage group : IIR 
Cultural requirement (property of mutant): resistant to cycloheximide (actidione) 

 

Locus Designation : acuD 
Linkage group : VR 
Enzyme (or gene function) : isocitrate lyase 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 
Corresponding mutation in Neurospora: acu-3 

 

Locus Designation : acuE 
Linkage group : IR 
Enzyme (or gene function) : malate synthase 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 
Corresponding mutation in Neurospora: acu-9 

 

Locus Designation : acuF 
Linkage group : VII 
Enzyme (or gene function) : PEP carboxykinase 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 
Corresponding mutation in Neurospora: acu-6 

 

Locus Designation : acuG 
Linkage group : VR 
Enzyme (or gene function) : fructose-1,6-diphosphatase 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 

 

Locus Designation : acuH 
Linkage group : V 
Enzyme (or gene function) : fructose-1,6-diphosphatase 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 

 

Locus Designation : acuJ 
Linkage group : IL 
Enzyme (or gene function) : fructose-1,6-diphosphatase 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 

 

Locus Designation : acuK 
Linkage group : IVR 
Enzyme (or gene function) : malic enzyme 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 

 

Locus Designation : acuL 
Linkage group : IVR 
Enzyme (or gene function) : malic enzyme 
Cultural requirement (property of mutant): cannot use acetate as a carbon source 

 

Locus Designation : adA 
Linkage group : IL 
Enzyme (or gene function) : adenylosuccinase 
Cultural requirement (property of mutant): requires adenine. No response to hypoxanthine. Response to adenine enhanced by addition of histidine 
Corresponding mutation in Neurospora: ad-4 

 

Locus Designation : adB 
Linkage group : VIIIR 
Enzyme (or gene function) : AMP succinate synthetase 
Cultural requirement (property of mutant): requires adenine. No response to hypoxanthine. Response to adenine enhanced by addition of histidine 
Corresponding mutation in Neurospora: ad-8 

 

Locus Designation : adC 
Linkage group : IIR 
Enzyme (or gene function) : AIR carboxylase 
Cultural requirement (property of mutant): requires adenine 
Corresponding mutation in Neurospora: ad-3A? 

 

Locus Designation : adD 
Linkage group : IIR 
Enzyme (or gene function) : AIR carboxylase 
Cultural requirement (property of mutant): requires adenine 
Corresponding mutation in Neurospora: ad-3B? 

 

Locus Designation : adE 
Linkage group : IR 
Cultural requirement (property of mutant): requires adenine 
Corresponding mutation in Neurospora: ad-7? 

 

Locus Designation : adF 
Linkage group : IR 
Cultural requirement (property of mutant): requires adenine 

 

Locus Designation : adG 
Linkage group : IL 
Cultural requirement (property of mutant): requires adenine 
Corresponding mutation in Neurospora: ad-6ad-9 

 

Locus Designation : adH 
Linkage group : IIL 
Cultural requirement (property of mutant): requires adenine 
Corresponding mutation in Neurospora: ad-2? 

 

Locus Designation : adI 
Linkage group : IIIL 
Cultural requirement (property of mutant): requires adenine, cannot use acetate as C source 

 

Locus Designation : agaA 
Linkage group : VIR 
Enzyme (or gene function) : arginase 
Cultural requirement (property of mutant): 
Corresponding mutation in Neurospora: aga 

 

Locus Designation : ahrA 
Linkage group : VIIIR 
Enzyme (or gene function) : asparaginase 
Cultural requirement (property of mutant): resistant to 5 mM aspartic hydroxamate on 10 mM nitrate 

 

Locus Designation : alX 
Linkage group : IIIL 
Enzyme (or gene function) : allantoinase 
Cultural requirement (property of mutant): cannot use allantoin as N source 
Corresponding mutation in Neurospora: aln-1 

 

Locus Designation : alcA 
Linkage group : VIIL 
Enzyme (or gene function) : alcohol dehydrogenase I 
Cultural requirement (property of mutant): 

 

Locus Designation : alcC 
Linkage group : VIIR 
Enzyme (or gene function) : alcohol dehydrogenase III 
Cultural requirement (property of mutant): 

 

Locus Designation : aldA 
Linkage group : VIIIR 
Enzyme (or gene function) : aldehyde dehydrogenase 
Cultural requirement (property of mutant): 

 

Locus Designation : anA 
Linkage group : IL 
Cultural requirement (property of mutant): requires aneurin (thiamine) 

 

Locus Designation : anB 
Linkage group : 
Cultural requirement (property of mutant): Requires aneurin (thiamine) 

 

Locus Designation : apnA 
Linkage group : IIL 
Enzyme (or gene function) : asparaginase II 
Cultural requirement (property of mutant): cannot use asparagine as a carbon source, barely uses it as a nitrogen source 

 

Locus Designation : argA 
Linkage group : VIR 
Enzyme (or gene function) : arginosuccinase 
Cultural requirement (property of mutant): requires arginine 
Corresponding mutation in Neurospora: arg-10 

 

Locus Designation : argB 
Linkage group : IIIL 
Enzyme (or gene function) : ornithine transcarbamylase 
Cultural requirement (property of mutant): responds to citrulline or arginine 
Corresponding mutation in Neurospora: arg-12 

 

Locus Designation : argC 
Linkage group : VIIIR 
Enzyme (or gene function) : arginosuccinate synthetase 
Cultural requirement (property of mutant): requires arginine 
Corresponding mutation in Neurospora: arg-1 

 

Locus Designation : argD 
Linkage group : V 
Cultural requirement (property of mutant): requires arginine 

 

Locus Designation : benA 
Linkage group : VIIIR 
Enzyme (or gene function) : beta-tubulin 
Cultural requirement (property of mutant): resistant to benomyl (Benlate) (approx. 10 ug/ml) 
Corresponding mutation in Neurospora: Bml 

 

Locus Designation : benB 
Linkage group : IIR 
Cultural requirement (property of mutant): resistant to benomyl (Benlate) (approx 2 ug/ml) 

 

Locus Designation : benC 
Linkage group : VIIR 
Cultural requirement (property of mutant): resistant to benomyl (Benlate) (approx 2 ug/ml) 

 

Locus Designation : biA1 
Linkage group : IR 
Cultural requirement (property of mutant): requires biotin 
Corresponding mutation in Neurospora: none 

 

Locus Designation : carA1 
Linkage group : VIIR 
Enzyme (or gene function) : ? 
Cultural requirement (property of mutant): resistant to 100 ppm carboxin
Sensitive strains (and not resistant ones) show a difference in sensitivity
associated with carbon source. In an acetate medium carboxin is 7 X as 
fungitoxic as on a glucose medium. VanTuyl (1975) Neth J Pl Path 81 122-123.

 

Locus Designation : carB2 
Linkage group :VIIR 
Enzyme (or gene function) : ? 
Cultural requirement (property of mutant): resistant to 100 ppm carboxin
Sensitive strains (and not resistant ones) show a difference in sensitivity
associated with carbon source. In an acetate medium carboxin is 7 X as 
fungitoxic as on a glucose medium. VanTuyl (1975) Neth J Pl Path 81 122-123.

 

Locus Designation :carC9 
Linkage group : 
Enzyme (or gene function) : 
Cultural requirement (property of mutant): resistant to 100 ppm carboxin
Sensitive strains (and not resistant ones) show a difference in sensitivity
associated with carbon source. In an acetate medium carboxin is 7 X as 
fungitoxic as on a glucose medium. VanTuyl (1975) Neth J Pl Path 81 122-123.

 

Locus Designation : choA 
Linkage group : VIIR 
Enzyme (or gene function) : phosphatidyl ethanolamine methyltransferase 
Cultural requirement (property of mutant): responds to choline 
Corresponding mutation in Neurospora: chol-1 

 

Locus Designation : choC 
Linkage group : VIIIR 
Cultural requirement (property of mutant): responds to choline 
Corresponding mutation in Neurospora: chol-2 

 

Locus Designation : cnxA 
Linkage group : VIIIR 
Enzyme (or gene function) : nitrate reductase, xanthine dehydrogenase 
Cultural requirement (property of mutant): cannot use nitrate as a nitrogen source 
Corresponding mutation in Neurospora: nit-7? 

 

Locus Designation : cnxB 
Linkage group : VIIIR 
Enzyme (or gene function) : nitrate reductase, xanthine dehydrogenase 
Cultural requirement (property of mutant): cannot use nitrate as a nitrogen source 
Corresponding mutation in Neurospora: nit-8? 

 

Locus Designation : cnxC 
Linkage group : VIIIR 
Enzyme (or gene function) : nitrate reductase, xanthine dehydrogenase 
Cultural requirement (property of mutant): cannot use nitrate as a nitrogen source 
Corresponding mutation in Neurospora: nit-9? 

 

Locus Designation : cnxE 
Linkage group : IIR 
Enzyme (or gene function) : nitrate reductase, xanthine dehydrogenase 
Cultural requirement (property of mutant): cannot use nitrate as a nitrogen source 
Corresponding mutation in Neurospora: nit-9? 

 

Locus Designation : cnxF 
Linkage group : VIIR 
Enzyme (or gene function) : nitrate reductase, xanthine dehydrogenase 
Cultural requirement (property of mutant): cannot use nitrate as a nitrogen source 
Corresponding mutation in Neurospora: nit-9? 

 

Locus Designation : cnxG 
Linkage group : VI 
Enzyme (or gene function) : nitrate reductase, xanthine dehydrogenase 
Cultural requirement (property of mutant): cannot use nitrate as a nitrogen source 
Corresponding mutation in Neurospora: nit-9? 

 

Locus Designation : cnxH 
Linkage group : IIIL 
Enzyme (or gene function) : nitrate reductase, xanthine dehydrogenase 
Cultural requirement (property of mutant): cannot use nitrate as a nitrogen source 
Corresponding mutation in Neurospora: nit-9? 

 

Locus Designation : cysA 
Linkage group : 
Enzyme (or gene function) : serine transacetylase 
Cultural requirement (property of mutant): supresses methG. Causes cysteine auxotrophy in combination with mecB or mecC 

 

Locus Designation : cysB 
Linkage group : 
Cultural requirement (property of mutant): Responds to cysteine, not to met or homocysteine when combined with mecB or mecC 

 

Locus Designation : cysC 
Linkage group : 
Cultural requirement (property of mutant): Responds to cysteine, not to met or homocysteine when combined with mecB or mecC 

 

Locus Designation : cysD 
Linkage group : 
Enzyme (or gene function) : homocysteine synthase 
Cultural requirement (property of mutant): combined with cysC, causes requirement for O-acetyl serine 

 

Locus Designation : drkA 
Linkage group : VIIR 
Cultural requirement (property of mutant): dark colored conidia 

 

Locus Designation : drkB 
Linkage group : IIR 
Cultural requirement (property of mutant): dark colored conidia, suppressor of brlA12 

 

Locus Designation : facA 
Linkage group : VR 
Enzyme (or gene function) : acetyl CoA synthetase 
Cultural requirement (property of mutant): Resistant to fluoroacetate, cannot use acetate as a carbon source 

 

Locus Designation : facB 
Linkage group : VIIIR 
Cultural requirement (property of mutant): fluoroacetate resistant, cannot use acetate as a carbon source 

 

Locus Designation : facC 
Linkage group : VIIIR 
Cultural requirement (property of mutant): fluoroacetate resistant, cannot use acetate as a carbon source 

 

Locus Designation : fpaA 
Linkage group : IL 
Cultural requirement (property of mutant): resistant to p-fluorophenylalanine, partial requirement for tyrosineresistance may be countered by excess phe 

 

Locus Designation : fpaB 
Linkage group : IL 
Cultural requirement (property of mutant): resistant to p-fluorophenylalanine, which may be countered by excess phe 

 

Locus Designation : fpaC 
Linkage group : VII 
Cultural requirement (property of mutant): resistant to p-fluorophenylalanine 

 

Locus Designation : fpaD 
Linkage group : III 
Cultural requirement (property of mutant): resistant to p-fluorophenylalanine 

 

Locus Designation : fpaI 
Linkage group : IL 
Cultural requirement (property of mutant): resistant to fluorophenylalanine 

 

Locus Designation : frA 
Linkage group : IVR 
Cultural requirement (property of mutant): Poor growth, as measured by colony size vs. wt on plates, with fructose or sucrose as sole C source 

 

Locus Designation : galA 
Linkage group : IIIL 
Enzyme (or gene function) : galactokinase 
Cultural requirement (property of mutant): poor growth, as measured by colony size vs. wt on plates, when galactose is the sole C source 

 

Locus Designation : galB 
Linkage group : II 
Cultural requirement (property of mutant): Grows more slowly than wt when galactose is the sole C source 

 

Locus Designation : galC 
Linkage group : VIII 
Cultural requirement (property of mutant): Grows more slowly than wt when galactose is sole C source 

 

Locus Designation : galD 
Linkage group : IL 
Enzyme (or gene function) : UDP-galactose pyrophosphorylase 
Cultural requirement (property of mutant): Poor growth, compared with wt on plates, when galactose is sole C source 

 

Locus Designation : galE 
Linkage group : IIIL 
Enzyme (or gene function) : galactokinase 
Cultural requirement (property of mutant): Poor growth, compared with wt on plates, when galactose is sole C source 

 

Locus Designation : galG 
Linkage group : VIIIR 
Cultural requirement (property of mutant): Cannot use galactose, suppresses brlA12 

 

Locus Designation : gamA 
Linkage group : I 
Cultural requirement (property of mutant): cannot use galactose, resistant to 33 mM molybdate on 10 mM ammonium tartrate 

 

Locus Designation : gamB 
Linkage group : VIIIR 
Cultural requirement (property of mutant): cannot use galactose, resistant to 33 mM molybdate on 10 mM ammonium tartrate 

 

Locus Designation : gamC 
Linkage group : ? 
Cultural requirement (property of mutant): cannot use galactose, resistant to 33 mM molybdate on 10 mM ammonium tartrate 

 

Locus Designation : gdhA 
Linkage group : IIIL 
Enzyme (or gene function) : NADP-glutamate dehydrogenase 
Cultural requirement (property of mutant): requires alanine or glutamine or other amino source 
Corresponding mutation in Neurospora: am 

 

Locus Designation : gdhB 
Linkage group : IV 
Enzyme (or gene function) : NAD-glutamate dehydrogenase 
Cultural requirement (property of mutant): grows poorly on 10 mM alanine as N source or 100 mM glutamate as sole N and C source 

 

Locus Designation : hisA 
Linkage group : IVL 
Enzyme (or gene function) : AICAR isomerase? 
Cultural requirement (property of mutant): Requires histidine 

 

Locus Designation : hisB 
Linkage group : I 
Enzyme (or gene function) : imidazole glycerophosphate dehydratase 
Cultural requirement (property of mutant): Requires histidine 
Corresponding mutation in Neurospora: his-1 

 

Locus Designation : hisC 
Linkage group : VIIIR 
Enzyme (or gene function) : imidazole acetol phosphate transaminase 
Cultural requirement (property of mutant): requires histidine 
Corresponding mutation in Neurospora: his-5 

 

Locus Designation : hisG 
Linkage group : II 
Enzyme (or gene function) : ATP phosphoribosyl pyrophosphorylase 
Cultural requirement (property of mutant): requires histidine 
Corresponding mutation in Neurospora: his-2 

 

Locus Designation : hisH 
Linkage group : VIIIR 
Enzyme (or gene function) : AICAR amidotransferase? 
Cultural requirement (property of mutant): requires histidine 

 

Locus Designation : hisJ 
Linkage group : VII 
Enzyme (or gene function) : regulatory? 
Cultural requirement (property of mutant): requires histidine 

 

Locus Designation : hxA 
Linkage group : VR 
Enzyme (or gene function) : xanthine dehydrogenase I 
Cultural requirement (property of mutant): cannot use hypoxanthine as a nitrogen source 
Corresponding mutation in Neurospora: xdh-1 

 

Locus Designation : hxB 
Linkage group : VII 
Enzyme (or gene function) : xanthine dehydrogenase I and II 
Cultural requirement (property of mutant): cannot use hypoxanthine as a nitrogen source 

 

Locus Designation : ileA 
Linkage group : IIR 
Enzyme (or gene function) : threonine dehydratase 
Cultural requirement (property of mutant): requires isoleucine 
Corresponding mutation in Neurospora: ile-1 

 

Locus Designation : imaA 
Linkage group : VIIR 
Cultural requirement (property of mutant): slight resistance to neomycin (4 mg/ml) and acriflavin (2 mg/ml) 

 

Locus Designation : imaB 
Linkage group : V 
Cultural requirement (property of mutant): resistant to chloramphenicol (5 mg/ml), hypersensitive to acriflavin (0.5 mg/ml) and cycloheximide (0.5 mg/ml) 

 

Locus Designation : imaC 
Linkage group : IIR 
Cultural requirement (property of mutant): resistant to chloramphenicol (5 mg/ml) and cycloheximide (2 mg/ml) 

 

Locus Designation : imaD 
Linkage group : VIIIR 
Cultural requirement (property of mutant): Hypersensitive to cycloheximide (0.5 mg/ml) 

 

Locus Designation : imaE 
Linkage group : II 
Cultural requirement (property of mutant): Resistant to chloramphenicol (5 mg/ml) 

 

Locus Designation : imaG 
Linkage group : III 
Cultural requirement (property of mutant): Resistant to cycloheximide (2 mg/ml) May be allelic with actA 

 

Locus Designation : imaH 
Linkage group : III 
Cultural requirement (property of mutant): Resistant to neomycin (4 mg/ml) 

 

Locus Designation : inoA 
Linkage group : IIR 
Cultural requirement (property of mutant): requires inositol 
Corresponding mutation in Neurospora: inl? 

 

Locus Designation : inoB 
Linkage group : IVR 
Cultural requirement (property of mutant): requires inositol 

 

Locus Designation : ivoA 
Linkage group : IIIL 
Cultural requirement (property of mutant): ivory colored conidiophores 

 

Locus Designation : ivoB 
Linkage group : VIIIR 
Enzyme (or gene function) : phenol oxidase 
Cultural requirement (property of mutant): ivory colored conidiophores 

 

Locus Designation : lacA 
Linkage group : VIR 
Cultural requirement (property of mutant): Forms normal size but diffuse colonies when lactose is sole C source 

 

Locus Designation : lacB 
Linkage group : IIR 
Cultural requirement (property of mutant): Forms normal size but diffuse colonies when lactose is sole C source 

 

Locus Designation : luA 
Linkage group : IL 
Cultural requirement (property of mutant): requires leucine 

 

Locus Designation : lysA 
Linkage group : VIR 
Cultural requirement (property of mutant): requires lysine 

 

Locus Designation : lysB 
Linkage group : VL 
Cultural requirement (property of mutant): requires lysine, slight response to aminoadipate 

 

Locus Designation : lysD 
Linkage group : VIIR 
Cultural requirement (property of mutant): responds to lysine or aminoadipate 

 

Locus Designation : lysE 
Linkage group : VR 
Cultural requirement (property of mutant): requires lysine 

 

Locus Designation : lysF 
Linkage group : IR 
Cultural requirement (property of mutant): responds to lysine or aminoadipate 

 

Locus Designation : malA 
Linkage group : 
Cultural requirement (property of mutant): Forms normal size but diffuse colonies when maltose is sole C source 

 

Locus Designation : manA 
Linkage group : VIIIR 
Enzyme (or gene function) : phosphomannose isomerase 
Cultural requirement (property of mutant): cannot use mannose or glucose as a carbon source, but does grow on 0.9% glucose + 0.1 % mannose 

 

Locus Designation : mauA 
Linkage group : IVR 
Enzyme (or gene function) : monoamine oxidase 
Cultural requirement (property of mutant): cannot use methylamine or ethanolamine as N sources 

 

Locus Designation : mauB 
Linkage group : IIR 
Enzyme (or gene function) : monoamine oxidase 
Cultural requirement (property of mutant): cannot use methylamine or ethanolamine as N sources 

 

Locus Designation : meaA 
Linkage group : IVR 
Cultural requirement (property of mutant): resistant to methylamine (100 mM) on nitrate media 
Corresponding mutation in Neurospora: mea-1 

 

Locus Designation : meaB 
Linkage group : IIIL 
Cultural requirement (property of mutant): resistant to methylamine (100 mM) on nitrate media 

 

Locus Designation : mecA 
Linkage group : IR 
Enzyme (or gene function) : cystathionine beta-synthase 
Cultural requirement (property of mutant): unable to grow on methionine + selenate 

 

Locus Designation : mecB 
Linkage group : IR 
Enzyme (or gene function) : gamma-cystathionase 
Cultural requirement (property of mutant): Unable to grow on methionine + selenate 

 

Locus Designation : mecC 
Linkage group : ? 
Enzyme (or gene function) : methionine adenosyltransferase 
Cultural requirement (property of mutant): unable to grow on methionine + selenate 
Corresponding mutation in Neurospora: eth-1 

 

Locus Designation : melA 
Linkage group : VII 
Cultural requirement (property of mutant): Excess melanin production 

 

Locus Designation : melB 
Linkage group : VIIR 
Cultural requirement (property of mutant): reduced melanin production 

 

Locus Designation : methB 
Linkage group : VIR 
Enzyme (or gene function) : cystathionine synthase 
Cultural requirement (property of mutant): responds to cystathionine, homocysteine or methionine 
Corresponding mutation in Neurospora: met-3,-7 

 

Locus Designation : methE 
Linkage group : VIIR 
Enzyme (or gene function) : Homoserine transacetylase 
Cultural requirement (property of mutant): requires methionine 
Corresponding mutation in Neurospora: met-5 

 

Locus Designation : methG 
Linkage group : IVL 
Enzyme (or gene function) : cystathionine B-lyase 
Cultural requirement (property of mutant): responds to homocysteine or methionine, may adapt to grow on minimal after 2-3 days 
Corresponding mutation in Neurospora: met-2 

 

Locus Designation : methH 
Linkage group : IIIL 
Enzyme (or gene function) : methyl tetrahydrofolate homocysteine transmethylase 
Cultural requirement (property of mutant): requires methionine, may adapt and grow on minimal after 2-3 days 
Corresponding mutation in Neurospora: met-8 

 

Locus Designation : niaD 
Linkage group : VIIIR 
Enzyme (or gene function) : nitrate reductase 
Cultural requirement (property of mutant): Cannot use nitrate as a nitrogen source, should be resistant to chlorate, unable to use glutamate as an N source 
Corresponding mutation in Neurospora: nit-3 

 

Locus Designation : nicA 
Linkage group : VL 
Cultural requirement (property of mutant): Requires nicotinic or anthranilic acid 

 

Locus Designation : nicB 
Linkage group : VIIR 
Cultural requirement (property of mutant): Requires nicotinic or anthranilic acid or tryptophan 
Corresponding mutation in Neurospora: nt? 

 

Locus Designation : nicC 
Linkage group : VIR 
Cultural requirement (property of mutant): Requires nicotinic acid 

 

Locus Designation : niiA 
Linkage group : VIIIR 
Enzyme (or gene function) : nitrite reductase 
Cultural requirement (property of mutant): Cannot use nitrite as a nitrogen source 
Corresponding mutation in Neurospora: nit-6 

 

Locus Designation : nirA 
Linkage group : VIIIR 
Enzyme (or gene function) : transcription activator? 
Cultural requirement (property of mutant): cannot use nitrite or nitrate 
Corresponding mutation in Neurospora: nit-4 

 

Locus Designation : oliA 
Linkage group : mito. 
Enzyme (or gene function) : ATP synthetase 
Cultural requirement (property of mutant): resistant to oligomycin 

 

Locus Designation : oliC 
Linkage group : VIIR 
Cultural requirement (property of mutant): resistant to oligomycin 
Corresponding mutation in Neurospora: oli 

 

Locus Designation : ornA 
Linkage group : IVR 
Enzyme (or gene function) : N-acetyl glutamate kinase + N-acetylglutamate semialdehyde dehydrogenase 
Cultural requirement (property of mutant): Requires ornithine, citrulline or arginine 
Corresponding mutation in Neurospora: arg-6 

 

Locus Designation : ornB 
Linkage group : VIIIR 
Enzyme (or gene function) : see ornA 
Cultural requirement (property of mutant): Requires ornithine, citrulline or arginine 
Corresponding mutation in Neurospora: arg-6 

 

Locus Designation : ornC 
Linkage group : IIIR 
Enzyme (or gene function) : N-acetyl ornithine deacetylase 
Cultural requirement (property of mutant): Requires ornithine, citrulline or arginine 
Corresponding mutation in Neurospora: arg-4 

 

Locus Designation : pabaA 
Linkage group : IR 
Cultural requirement (property of mutant): Requires p-aminobenzoate 

 

Locus Designation : pabaB 
Linkage group : IVR 
Cultural requirement (property of mutant): Requires p-aminobenzoate 

 

Locus Designation : pacA 
Linkage group : IV 
Enzyme (or gene function) : acid phosphatase 
Cultural requirement (property of mutant): phosphatase activity lacking at pH 4.8, normal at pH 8.3 

 

Locus Designation : pacC 
Linkage group : VIR 
Enzyme (or gene function) : acid phosphatase 
Cultural requirement (property of mutant): phosphatase activity is reduced at pH 4.8 (some activity at 25 degrees, none at 35), normal at pH 8.2 
Corresponding mutation in Neurospora: pho-3? 

 

Locus Designation : palA 
Linkage group : IIIL 
Enzyme (or gene function) : alkaline phosphatase 
Cultural requirement (property of mutant): phosphatase activity is lacking at pH 8.2, but is greater than wt at pH 4.8 
Corresponding mutation in Neurospora: pho-2? 

 

Locus Designation : palB 
Linkage group : VIIR 
Enzyme (or gene function) : alkaline phosphatase 
Cultural requirement (property of mutant): phosphatase activity is generally lacking at pH 8.2 but is greater than normal at pH 4.8 
Corresponding mutation in Neurospora: pho-2? 

 

Locus Designation : palC 
Linkage group : IVR 
Enzyme (or gene function) : alkaline phosphatase 
Cultural requirement (property of mutant): phosphatase activity is lacking at pH 8.2 but is greater than wt at pH 4.8 
Corresponding mutation in Neurospora: pho-2? 

 

Locus Designation : palD 
Linkage group : VIIR 
Enzyme (or gene function) : alkaline phosphatase 
Cultural requirement (property of mutant): phosphatase activity is lacking at pH 8.3 but normal at pH 4.8 
Corresponding mutation in Neurospora: pho-2? 

 

Locus Designation : palE 
Linkage group : VIIIL 
Enzyme (or gene function) : alkaline phosphatase 
Cultural requirement (property of mutant): phosphatase activity is lacking at pH 8.3 but is greater than wt at pH 4.8 
Corresponding mutation in Neurospora: pho-2? 

 

Locus Designation : palF 
Linkage group : VIIR 
Enzyme (or gene function) : alkaline phosphatase 
Cultural requirement (property of mutant): phosphatase activity is lacking at pH 8.3 but is greater than wt at pH 4.8 
Corresponding mutation in Neurospora: pho-2? 

 

Locus Designation : palcA 
Linkage group : IIL 
Enzyme (or gene function) : acid and alkaline phosphatase 
Cultural requirement (property of mutant): no phosphatase activity at pH 4.8 or 8.3 Shows partial activity at either pH at 25 degrees 
Corresponding mutation in Neurospora: nuc-1? 

 

Locus Designation : palcC 
Linkage group : VIIIR 
Enzyme (or gene function) : acid and alkaline phosphatase 
Cultural requirement (property of mutant): phosphatase activity is lacking at pH 4.8 and greatly reduced at pH 8.3 
Corresponding mutation in Neurospora: nuc-1? 

 

Locus Designation : pantoA 
Linkage group : VIIIR 
Enzyme (or gene function) : pantothenate synthetase 
Cultural requirement (property of mutant): Requires pantothenate 
Corresponding mutation in Neurospora: pan-1? 

 

Locus Designation : pantoB 
Linkage group : VIIR 
Cultural requirement (property of mutant): Requires pantothenate 

 

Locus Designation : pantoC 
Linkage group : IIIL 
Cultural requirement (property of mutant): Requires pantothenate 

 

Locus Designation : pdhA 
Linkage group : IR 
Enzyme (or gene function) : lipoate acetyltransferase 
Cultural requirement (property of mutant): Requires acetate, may be inhibited by sucrose 
Corresponding mutation in Neurospora: ace-4 

 

Locus Designation : pdhB 
Linkage group : VL 
Enzyme (or gene function) : beta subunit of pyruvate decarboxylase 
Cultural requirement (property of mutant): requires acetate, may be inhibited by sucrose 
Corresponding mutation in Neurospora: ace-2,-3 

 

Locus Designation : pdhC 
Linkage group : VIIIL 
Enzyme (or gene function) : alpha subunit of pyruvate decarboxylase 
Cultural requirement (property of mutant): requires acetate, may be inhibited by sucrose 
Corresponding mutation in Neurospora: ace-3,-2 

 

Locus Designation : phenA 
Linkage group : IIIR 
Enzyme (or gene function) : Prephenate dehydratase 
Cultural requirement (property of mutant): Requires phenylalanine 
Corresponding mutation in Neurospora: phe-2 

 

Locus Designation : phenB 
Linkage group : VIIR 
Cultural requirement (property of mutant): Has a partial requirement for phenylalanine 

 

Locus Designation : pkiA 
Linkage group : V 
Enzyme (or gene function) : pyruvate kinase 
Cultural requirement (property of mutant): Require acetate or alanine as a carbon source 
Corresponding mutation in Neurospora: ace-8 

 

Locus Designation : pppA 
Linkage group : VIII 
Enzyme (or gene function) : transaldolase 
Cultural requirement (property of mutant): Cannot use xylose or arabinose as carbon sources 

 

Locus Designation : pppB 
Linkage group : III 
Cultural requirement (property of mutant): Cannot use xylose or arabinose as carbon sources 

 

Locus Designation : proA 
Linkage group : IL 
Cultural requirement (property of mutant): Responds best to proline; also to ornithine, citrulline or arginine 
Corresponding mutation in Neurospora: pro-3 

 

Locus Designation : proB 
Linkage group : IL 
Cultural requirement (property of mutant): Responds best to proline; also to ornithine, citrulline or arginine 
Corresponding mutation in Neurospora: pro-4 

 

Locus Designation : puA 
Linkage group : IIR 
Enzyme (or gene function) : ornithine decarboxylase 
Cultural requirement (property of mutant): Requires putrescine 
Corresponding mutation in Neurospora: spe-1 

 

Locus Designation : punA 
Linkage group : IIR 
Cultural requirement (property of mutant): Grows poorly on putrescine as a nitrogen source 

 

Locus Designation : pycA 
Linkage group : V 
Enzyme (or gene function) : pyruvate carboxylase 
Cultural requirement (property of mutant): Prefers acetate or glutamate + fructose as carbon sources 
Corresponding mutation in Neurospora: suc 

 

Locus Designation : pyrD 
Linkage group : VIIIR 
Enzyme (or gene function) : dehydro-orotase 
Cultural requirement (property of mutant): Responds to uridine 
Corresponding mutation in Neurospora: pyr-6 

 

Locus Designation : pyrE 
Linkage group : IL 
Enzyme (or gene function) : dehydro-orotate dehydrogenase 
Cultural requirement (property of mutant): Responds to uridine 
Corresponding mutation in Neurospora: pyr-1 

 

Locus Designation : pyrG 
Linkage group : IL 
Enzyme (or gene function) : orotidine monophosphate decarboxylase 
Cultural requirement (property of mutant): Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil 
Corresponding mutation in Neurospora: pyr-4 

 

Locus Designation : pyroA 
Linkage group : IVR 
Cultural requirement (property of mutant): Responds to pyridoxine 

 

Locus Designation : pyroB 
Linkage group : IL 
Cultural requirement (property of mutant): Responds to pyridoxine 
Corresponding mutation in Neurospora: pdx-1? 

 

Locus Designation : rA 
Linkage group : IL 
Cultural requirement (property of mutant): phosphatase activity is normal at pH 4.8 but greater than wt at pH 8.3 

 

Locus Designation : riboA1 
Linkage group : IL 
Cultural requirement (property of mutant): Requires riboflavin 

 

Locus Designation : riboB 
Linkage group : VIIIR 
Cultural requirement (property of mutant): Requires riboflavin. Confers self-sterility 

 

Locus Designation : riboC 
Linkage group : V 
Cultural requirement (property of mutant): Requires riboflavin 

 

Locus Designation : riboD 
Linkage group : VR 
Cultural requirement (property of mutant): requires riboflavin on nitrate media 

 

Locus Designation : riboE 
Linkage group : IIR 
Cultural requirement (property of mutant): requires riboflavin 

 

Locus Designation : riboF 
Linkage group : I 
Cultural requirement (property of mutant): Requires riboflavin 

 

Locus Designation : sA 
Linkage group : IIIL 
Enzyme (or gene function) : PAPS reductase 
Cultural requirement (property of mutant): responds to sulfite, resistant to 0.5 mM selenate 
Corresponding mutation in Neurospora: cys-5 

 

Locus Designation : sB 
Linkage group : VIR 
Enzyme (or gene function) : deficient in sulfate uptake 
Cultural requirement (property of mutant): resistant to 10 mM chromate, 1 mM selenate 
Corresponding mutation in Neurospora: cys-12 

 

Locus Designation : sC 
Linkage group : IIIL 
Enzyme (or gene function) : ATP sulfurylase 
Cultural requirement (property of mutant): responds to sulfite, resistant to 1 mM selenate on medium containing 0.1 mM methionine 
Corresponding mutation in Neurospora: cys-11 

 

Locus Designation : sD 
Linkage group : VIIIR 
Enzyme (or gene function) : adenosine 5 phosphosulfate kinase 
Cultural requirement (property of mutant): responds to sulfite, more sensitive than wt to 0.25 mM selenate 

 

Locus Designation : sE 
Linkage group : VIIIR 
Enzyme (or gene function) : PAPS reductase 
Cultural requirement (property of mutant): responds to sulfite, more sensitive than wt to 0.25 mM selenate 
Corresponding mutation in Neurospora: cys-5 

 

Locus Designation : sF 
Linkage group : VIIR 
Cultural requirement (property of mutant): responds to thiosulfate, leaky 

 

Locus Designation : sbA 
Linkage group : VIR 
Cultural requirement (property of mutant): Poor growth, as measured colony size vs wt on plates, on sorbitol as sole C source 

 

Locus Designation : sltA 
Linkage group : VIR 
Cultural requirement (property of mutant): Inhibited by 0.5 M NaCl in medium 

 

Locus Designation : sorA 
Linkage group : I 
Cultural requirement (property of mutant): Resistant to sorbose when glycerol is C source, also resistant to deoxyglucose 

 

Locus Designation : sorB 
Linkage group : IIIL 
Enzyme (or gene function) : phosphoglucomutase 
Cultural requirement (property of mutant): forms small colonies 
Corresponding mutation in Neurospora: rg-1? 

 

Locus Designation : spsA 
Linkage group : III 
Cultural requirement (property of mutant): Can use 0.06 mM spermidine in place of putrescine, but is inhibited by 6 mM spermidine 

 

Locus Designation : stuA 
Linkage group : IL 
Cultural requirement (property of mutant): stunted conidiophores 

 

Locus Designation : sucA 
Linkage group : ? 
Cultural requirement (property of mutant): cannot use succinate as a carbon source 

 

Locus Designation : sulA 
Linkage group : IL 
Cultural requirement (property of mutant): resistant to sulphanilamide 

 

Locus Designation : tamA 
Linkage group : VI 
Cultural requirement (property of mutant): resistant to 5 mM thiourea or 0.32 mM aspartate hydroxamate when 10 mM alanine is the N source 

 

Locus Designation : telA 
Linkage group : VII 
Cultural requirement (property of mutant): mound shaped colony 

 

Locus Designation : thiA 
Linkage group : IIR 
Cultural requirement (property of mutant): Responds to thiazole 
Corresponding mutation in Neurospora: thi-3? 

 

Locus Designation : trypA 
Linkage group : IIR 
Enzyme (or gene function) : anthranilate synthetase 
Cultural requirement (property of mutant): responds to anthranilate, indole or tryptophan 
Corresponding mutation in Neurospora: trp-2 

 

Locus Designation : trypB 
Linkage group : IL 
Enzyme (or gene function) : tryptophan synthetase 
Cultural requirement (property of mutant): Requires tryptophan 
Corresponding mutation in Neurospora: trp-3 

 

Locus Designation : trypC 
Linkage group : VIIIR 
Enzyme (or gene function) : Anthranilate synthatase, phosphoribosyl transferase, IGP synthetase 
Cultural requirement (property of mutant): some respond to anthranilate, others only to indole or tryptophan Optimal concentration of tryptophan is 4 mM 
Corresponding mutation in Neurospora: trp-1 

 

Locus Designation : trypD 
Linkage group : IIR 
Enzyme (or gene function) : anthranilate phosphoribosyl transferase 
Cultural requirement (property of mutant): responds to indole or tryptophan 
Corresponding mutation in Neurospora: trp-4 

 

Locus Designation : uaX 
Linkage group : VI 
Cultural requirement (property of mutant): slow growth when uric acid is the N source 

 

Locus Designation : uaY 
Linkage group : VIIIR 
Enzyme (or gene function) : xanthine dehydrogenase and urate oxidase regulation 
Cultural requirement (property of mutant): Cannot use uric acid as N source 

 

Locus Designation : uaZ 
Linkage group : IL 
Enzyme (or gene function) : urate oxidase 
Cultural requirement (property of mutant): 

 

Locus Designation : uapA 
Linkage group : IR 
Enzyme (or gene function) : uric acid-xanthine permease 
Cultural requirement (property of mutant): 

 

Locus Designation : ureA 
Linkage group : VIIIR 
Enzyme (or gene function) : urea uptake 
Cultural requirement (property of mutant): Cannot use urea as N source, resistant to thiourea 

 

Locus Designation : ureB 
Linkage group : VIIIR 
Enzyme (or gene function) : urease 
Cultural requirement (property of mutant): Cannot use urea as N source, no urease activity 
Corresponding mutation in Neurospora: ure-2 

 

Locus Designation : ureC 
Linkage group : VII 
Enzyme (or gene function) : urease 
Cultural requirement (property of mutant): Cannot use urea as N source, no urease activity 
Corresponding mutation in Neurospora: ure-3 

 

Locus Designation : ureD 
Linkage group : VIIIR 
Enzyme (or gene function) : urease 
Cultural requirement (property of mutant): Cannot use urea as N source. No urease activity 
Corresponding mutation in Neurospora: ure-4 

 

Locus Designation : wA 
Linkage group : IIL 
Cultural requirement (property of mutant): white conidia 

 

Locus Designation : wetA 
Linkage group : VIIR 
Cultural requirement (property of mutant): white conidia autolyze, giving wet appearance 

 

Locus Designation : xprD 
Linkage group : IIIR 
Enzyme (or gene function) : regulation of nitrogen metabolism 
Cultural requirement (property of mutant): =areA 
Corresponding mutation in Neurospora: nit-2 

 

Locus Designation : yA 
Linkage group : IR 
Enzyme (or gene function) : p-diphenol oxidase 
Cultural requirement (property of mutant): yellow conidia 

Year prepared: 

1970