Exoproteome profile of Aspergillus fumigatus isolates from fungal keratitis patients

R. Sivasamv1*. Namperumalsamy Venkatesh Prajna2 .Prajna Lalitha3, ChitraThangavel1 and Kuppamuthu Dharmalingam1

Abstract: 

Fungal keratitis is a major cause of vision loss and blindness. Aspergillus fumigatus is one of the pathogenic fungi, which causes keratitis. Exoproteome is a part of a proteome consisting of proteins secreted from a cell. Several secreted proteins are known to be involved in defense mechanisms or immune evasion, thus, probably contributing to pathogenicity.In this study we examined the exoproteome profile of A. fumigatus clinical isolates and ATCC culture grown in Aspergillus minimal medium (AMM) were analysed in order to determine the possible virulence associated proteins by comparative proteomics.Clinical isolates were identified and confirmed as A. fumigatus by molecular methods including ITS gene sequenceing and rod A gene single nucleotide polymorphism. Confirmed isolates were taken for exoproteome analysis. The exoproteome profiling of A.fumigatus clinical isolates and ATCC 204305 was performed using ID SDS-PAGE and Massspectrometry. On analyzing the exoproteome profile, differential expression of 14 proteins was observed among the clinical isolates as well as between ATCC 204305 and clinical isolates. Out of 14 proteins identified, two proteins MnSOD and FG-GAP protein were found to be differentially regulated in the clinical isolates compared to ATCC 204305. Further, upregulation of MnSOD protein was confirmed at mRNA level by real-time RT-qPCR and zymogram analysis. Differential expression of MnSOD in the clinical isolate indicates a defense mechanism seen in clinical isolates. ‘Corresponding author E-mail:[email protected]
2014

abstract No: 

OP-017

Full conference title: 

Society for Indian Human and Animal Mycologists 2014